Image it green hypoxia reagent
Web21 feb. 2024 · Then, Image-iT™ Green Hypoxia Reagent stock solution (2.5 µM) was added to the cells, and then incubated for 30 min. The cells were then stained with DAPI for 10 min and imaged using LCSM. The immunofluorescence of HIF-1α was conducted to value the hypoxia of cells. Cells were inoculated on the coverslip. WebBlue: Hoechst 33342; Green: SYTOX™ green; Red: Image-iT™ Red Hypoxia Reagent (d) Schematic and (e-f) fluorescence microscopy of the co-culture of the spheroid with an endothelial network of ...
Image it green hypoxia reagent
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Web26 mei 2024 · It has been reported that the probe Image-iT Green Hypoxia Reagent is fluorogenic when atmospheric oxygen levels are lower than 5% (Stone et al., 2024). … WebImage-IT Green Hypoxia Reagent was excited at 488 nm (detection: 496–550 nm), CellTrace Violet at 405 nm (detection: 413–460 nm), and CytoTell Red at 647 nm (detection: 656–750 nm). 2-NBDG was excited at 467 nm (detection: ... the hypoxia sensor dye Image-IT was added and the oxygen concentration throughout the device characterized.
WebImage-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells. It is non-fluorescent when live cells are in an environment with normal oxygen concentrations and becomes fluorescent when oxygen levels are decreased. Web7 sep. 2024 · Restoring sufficient vascularity of the ischemia/hypoxia flap is always the critical issue in flap surgeries. In a previous studies microRNA-21 (miR-21) expression was upregulated after rat skin flap surgery. MiR-21 has been reported to be induced by hypoxia and the function of miR-21 involves in the process of angiogenesis. However, the precise …
WebFigure 1: Image-iT Hypoxia Green Reagent – Spectra Hypoxia CONCLUSIONS Hypoxia is a very important phenomenon in many human diseases. Piminidazoles work well at ≤ … WebImage-iT Green Hypoxia Reagent is a fluorogenic compound that is live cell permeable and becomes fluorescent in environments with low oxygen concentrations. These properties …
Web24 okt. 2024 · The image-iT reagent can be used in conjunction with our system to detect cells that are currently hypoxic (Image-iT+ /GFP+) and those that have been previously exposed to hypoxia but...
Web2 apr. 2024 · The CCK-8 kit (Dojindo) was used to detect cell viability. Before hypoxia treatment, 10,000 cells were planted in each well of a 96-well plate, and three holes were made in each well. After 24 h of culture, the medium was replaced and 10 μL of the CCK-8 reagent was added to each well (incubation, 37°C, 2 h). town and regional planning salary in saWebImage-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells. It is non-fluorescent when live cells are in an environment with normal oxygen concentrations and becomes … town and regional planning act namibiaWebImage-iT Green Hypoxia Reagentは、生細胞中の低酸素状態を測定するための、固定可能な新しい蛍光性化合物です。 生細胞が正常な酸素濃度環境にある場合は無蛍光で、酸素濃度が低下すると蛍光を発します。 Image-iT Green Hypoxia Reagentは、細胞／組織が正常な酸素濃度に戻っても蛍光は持続するため、蛍光シグナルの損失を最小限に抑えなが … powerchute unable to carry out a self-testWeb11 apr. 2024 · HIF-1α activates TMEM237 transcription in hypoxic HCC cells. We previously investigated hypoxia-induced transcripts in Hep3B cells using a microarray assay [].The microarray data (GSE155505) showed that TMEM237 was significantly upregulated in Hep3B cells under hypoxic conditions (Fig. 1a). Then, RT‒qPCR and Western blotting … town and regional planning upWeb22 okt. 2024 · Bottom: Fluorescence signal from Image-iT Green Hypoxia Reagent. Scale bars 200 µm. Full size image. After hypoxia, the uptake of nanoparticles was clearly enhanced ... powerchute versionWebImage-iT Green Hypoxia Reagent is a novel, fixable fluorogenic compound for measuring hypoxia in live cells. Brand: Invitrogen™ I14833 Additional Details : Weight : 0.01750kg View more versions of this product Product Code. 15940773 4400.00 SEK / Pack of 5 Quantity In Stock to see stock. Add to basket Description Specifications Documents powerchute ups 通信切断Web25 okt. 2024 · A Fluorescence imaging of cells after the zebrafish cell line (ZF4) was cultured on the platform of dissolved oxygen gradient for 6 h and stained with cellular oxygen probe Image-iT™ Green Hypoxia Reagent. λ excitation = 488 nm, λ emission = 500–600 nm. B Fluorescence density quantification of images in A. powerchute v3.1.0